IHC

PRAME

Preferentially expressed antigen in melanoma (PRAME) is detected in approximately 90% of melanoma subtypes, while it is negative in roughly 85% of cutaneous melanocytic nevi. Immunohistochemical detection of PRAME is useful as an adjunct diagnostic tool to help distinguish malignant melanoma from benign melanocytic lesions.

Synonyms

Preferentially Expressed Antigen in Melanoma

Full Description

Preferentially expressed antigen in melanoma (PRAME) is detected in approximately 90% of melanoma subtypes, while it is negative in roughly 85% of cutaneous melanocytic nevi. Immunohistochemical detection of PRAME is useful as an adjunct diagnostic tool to help distinguish malignant melanoma from benign melanocytic lesions.

Methodology

IHC

Specimen Requirements

Tissue Block: Formalin-fixed, paraffin-embedded (FFPE) tumor block preferred. Unstained Slides: Recommended 2 charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked. Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.

Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

Turnaround Time

24 hrs

CPT Codes

88342x1 or 88341x1