Molecular

PML-RARA Translocation

Real-time RT-PCR assay for quantitative detection of the t(15;17) PML-RARA genes translocation, found in the vast majority of acute promyelocytic leukemia (APL). Positive results are quantified as a ratio between the abnormal isoform and the normal control gene.Identification of these fusion transcripts is closely correlated with responsiveness to treatment, improvement of survival, monitoring minimal residual disease and predicting relapse.

Synonyms

t(15;17)

Full Description

Real-time RT-PCR assay for quantitative detection of the t(15;17) PML-RARA genes translocation, found in the vast majority of acute promyelocytic leukemia (APL). Positive results are quantified as a ratio between the abnormal isoform and the normal control gene.Identification of these fusion transcripts is closely correlated with responsiveness to treatment, improvement of survival, monitoring minimal residual disease and predicting relapse.

Methodology

Molecular

Specimen Requirements

Whole blood (3-5 mL), Bone marrow (1-2 mL), in Lavender-top (EDTA) tube.

Storage and Transportation

Use cold pack for transport but not in direct contact with specimen. Ship same day as drawn whenever possible.

Turnaround Time

7 days

CPT Codes

81315