IHC

Perforin

Identifies a cytolytic protein stored in the granules of cytotoxic T lymphocytes (CTLs), which enables these cells to destroy targets presenting foreign antigens. Perforin is highly expressed in CD8⁺ T cells and, to a lesser degree, in γ/δ T cells and NK cells. It can be useful for detecting activated CTLs in conditions such as severe graft-versus-host disease, chronic renal rejection, peripheral T-cell lymphomas, and in identifying NK-cell lymphomas.

Full Description

Identifies a cytolytic protein stored in the granules of cytotoxic T lymphocytes (CTLs), which enables these cells to destroy targets presenting foreign antigens. Perforin is highly expressed in CD8⁺ T cells and, to a lesser degree, in γ/δ T cells and NK cells. It can be useful for detecting activated CTLs in conditions such as severe graft-versus-host disease, chronic renal rejection, peripheral T-cell lymphomas, and in identifying NK-cell lymphomas.

Methodology

IHC

Specimen Requirements

Tissue Block: Formalin-fixed, paraffin-embedded (FFPE) tumor block preferred. Unstained Slides: Recommended 2 charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked. Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.

Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

Turnaround Time

24 Hrs

CPT Codes

88342x1 or 88341x1