IHC

P57

Identifies p57, a paternally imprinted cell-cycle inhibitor that suppresses G1 cyclin activity and regulates cell proliferation. Loss of p57 expression occurs in the cytotrophoblasts of complete molar pregnancies. The p57 gene, located on chromosome 11p15.5, is a recognized tumor-suppressor candidate implicated in disorders such as sporadic cancers, Wilms tumor, and Beckwith-Wiedemann syndrome. Evaluation of p57 expression combined with ploidy analysis (e.g., FISH) aids in distinguishing complete hydatidiform moles (absent nuclear p57) from partial moles and non-molar hydropic abortions (normal expression).

Synonyms

Kip2, CDKN 2C

Full Description

Identifies p57, a paternally imprinted cell-cycle inhibitor that suppresses G1 cyclin activity and regulates cell proliferation. Loss of p57 expression occurs in the cytotrophoblasts of complete molar pregnancies. The p57 gene, located on chromosome 11p15.5, is a recognized tumor-suppressor candidate implicated in disorders such as sporadic cancers, Wilms tumor, and Beckwith-Wiedemann syndrome. Evaluation of p57 expression combined with ploidy analysis (e.g., FISH) aids in distinguishing complete hydatidiform moles (absent nuclear p57) from partial moles and non-molar hydropic abortions (normal expression).

Methodology

IHC

Specimen Requirements

Tissue Block: Formalin-fixed, paraffin-embedded (FFPE) tumor block preferred. Unstained Slides: Recommended 2 charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked. Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.

Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

Turnaround Time

24 Hrs

CPT Codes

88342x1 or 88341x1