IHC

MSH6

Identifies one of the mismatch repair gene products. MSH6 mutations appear to be associated with atypical HNPCC and in particular with development of endometrial carcinoma or atypical endometrial hyperplasia, the presumed precursor of endometrial cancer. Loss of expression can help identify tumors in Lynch Syndrome patients, as well as identify MSI type sporadic colonic and other carcinomas. Loss of MSG6 is generally accompanied by simultaneous loss of MSH2, although selective loss of MSH6 can also be seen.

Full Description

Identifies one of the mismatch repair gene products. MSH6 mutations appear to be associated with atypical HNPCC and in particular with development of endometrial carcinoma or atypical endometrial hyperplasia, the presumed precursor of endometrial cancer. Loss of expression can help identify tumors in Lynch Syndrome patients, as well as identify MSI type sporadic colonic and other carcinomas. Loss of MSG6 is generally accompanied by simultaneous loss of MSH2, although selective loss of MSH6 can also be seen.

Methodology

IHC

Specimen Requirements

Tissue Block: Formalin-fixed, paraffin-embedded (FFPE )tumor block preferred. Unstained Slides: Recommended 2 charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked. Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.

Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

Turnaround Time

Global: 48 Hrs, Image Analysis (tech-only): 36 Hrs, Tech-Only (stain only): 24 Hrs

CPT Codes

88342x1 or 88341x1; 88360x1 or 88361x1