IHC

CD5

A cell surface protein expressed on most thymocytes, immature peripheral T cells, and nearly all mature T cells, but not on NK cells. Also stains normal mantle zone B cells in lymph nodes and spleen, and B cells in pleural and peritoneal cavities. Aberrant expression occurs in certain B-cell neoplasms, including CLL/SLL, mantle cell lymphoma, and hairy cell leukemia, as well as in most T-cell malignancies and thymic carcinomas. Typically negative in spindle cell thymoma. In fetuses, most B cells in spleen and cord blood are CD5 positive.

Full Description

A cell surface protein expressed on most thymocytes, immature peripheral T cells, and nearly all mature T cells, but not on NK cells. Also stains normal mantle zone B cells in lymph nodes and spleen, and B cells in pleural and peritoneal cavities. Aberrant expression occurs in certain B-cell neoplasms, including CLL/SLL, mantle cell lymphoma, and hairy cell leukemia, as well as in most T-cell malignancies and thymic carcinomas. Typically negative in spindle cell thymoma. In fetuses, most B cells in spleen and cord blood are CD5 positive.

Methodology

IHC

Specimen Requirements

Tissue Block: Formalin-fixed, paraffin-embedded (FFPE) tumor block preferred. Unstained Slides: Recommended 2charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked. Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.

Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

Turnaround Time

24 hrs

CPT Codes

88342x1 or 88341x1